Takashima, Hiroshi published the artcileSynthesis and spectroscopic properties of reconstituted zinc-myoglobin appending a DNA-binding platinum(II) complex, COA of Formula: C17H19BrN2O2, the main research area is preparation myoglobin zinc protoporphyrin derivative platinum bipyridine complex; DNA binding myoglobin zinc protoporphyrin derivative platinum bipyridine complex; fluorescence myoglobin zinc protoporphyrin derivative platinum bipyridine complex.
Apo-myoglobin (apo-Mb) was reconstituted with a zinc-porphyrin derivative linked via an ethylenediamine derivative to a DNA-binding Pt complex, [Pt(bpy)(μ-enPP)Zn]Cl2 (μ-enPP = protoporphyrin derivative linked to ethylenediamine, bpy = 2,2′-bipyridine). The steady-state fluorescence of the cofactor, [Pt(bpy)(μ-enPP)Zn]Cl2, in MeOH indicates that the excited singlet state of zinc-porphyrin was almost quenched, probably because of the strong hydrophobic and π-π stacking interactions between the [Pt(bpy)(μ-enPP)Zn]2+ ions. In the reconstituted Mb-[Pt(bpy)(μ-enPP)Zn]2+, the quenching reaction of 1(ZnMb)* with the [Pt(bpy)(en)]2+ moiety does not occur, indicating apo-Mb matrix is essential. However, when the [Pt(bpy)(en)]2+ moiety was excited, the enhancement of the fluorescence from ZnMb unit was observed It is suggested that the energy transfer from 1([Pt(bpy)(en)]2+)* to ZnMb occurs. The spectroscopic changes of ZnMb-[Pt(bpy)(en)]2+ in the presence of calf-thymus DNA were also studied. Soret band at 428 nm gradually decreased, and isosbestic points at 321, 414, and 432 nm were observed with increasing DNA concentration When the PtII moiety was excited at λex 321 nm, the fluorescence signal around 600 nm similarly decreased. The synthetic modification of ZnMb with a DNA-binding PtII complex demonstrates sensitive fluorescent signal for DNA detection and valuable information to study photoinduced electron transfer within a Mb-DNA complex.
Chemistry & Biodiversity published new progress about Fluorescence. 352351-55-4 belongs to class bromides-buliding-blocks, name is (9H-Fluoren-9-yl)methyl (2-aminoethyl)carbamate hydrobromide, and the molecular formula is C17H19BrN2O2, COA of Formula: C17H19BrN2O2.
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary