Obeid, M.;Allous, I.;Hirbali, J. published 《Validated RP-HPLC method for determination of bromhexine hydrochloride, terbutaline sulfate and guaiphenesine in pharmaceutical dosage forms》 in 2016. The article was appeared in 《Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry》. They have made some progress in their research.Safety of 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride The article mentions the following:
A simple and accurate reversed phase HPLC method for simultaneous estimation of Syrian cough syrup containing Bromhexine Hydrochloride, Terbutaline Sulfate, and Guaiphenesin was developed. Separations were carried out on Column: C18(1005) (25 × 4.6mm, with precolumn). The mobile phase was methanol: buffer (600:400 volume/volume). The elution of the analytes was achieved in less than 10 min with a flow rate of 1.5 mL/min. Detection was by using UV absorbance at a wavelength of 276 nm. Different anal. performance parameters such as linearity, precision, accuracy, and robustness were determined and found in the acceptance range of American pharmacopoeia (USP34). To complete the study, the researchers used 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride (cas: 611-75-6) .
2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride(cas: 611-75-6) (BHH; 250μM; 24 hours) also significantly attenuates HGF-induced invasion of LNCaP and C4-2B cells that natively express TMPRSS2. No significant toxicity is observed over a 48-hour period exposing LNCaP, DU145, PC3, or HepG2 cells to Bromhexine hydrochloride concentrations ranging from 0μM to 250μM. Bromhexine hydrochloride exposure does not induce cell death or substantially suppress the growth of DU145 cells.Bromhexine hydrochloride (20 μM; 48 h) inhibits dendritic cells infection with HIV-1.Safety of 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride
Reference:
Bromide – Wikipedia,
bromide – Wiktionary