Category: 352351-55-4

Sun, Xiaoli published the artcileDevelopment of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging, Computed Properties of 352351-55-4, the main research area is SNAP tag fluorogenic probe wash free fluorescence imaging.

The ability to specifically attach chem. probes to individual proteins represents a powerful approach to the study and manipulation of protein function in living cells. It provides a simple, robust and versatile approach to the imaging of fusion proteins in a wide range of exptl. settings. However, a potential drawback of detection using chem. probes is the fluorescence background from unreacted or nonspecifically bound probes. In this report the authors present the design and application of novel fluorogenic probes for labeling SNAP-tag fusion proteins in living cells. SNAP-tag is an engineered variant of the human repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) that covalently reacts with benzylguanine derivatives Reporter groups attached to the benzyl moiety become covalently attached to the SNAP tag while the guanine acts as a leaving group. Incorporation of a quencher on the guanine group ensures that the benzylguanine probe becomes highly fluorescent only upon labeling of the SNAP-tag protein. The authors describe the use of intramolecularly quenched probes for wash-free labeling of cell surface-localized epidermal growth factor receptor (EGFR) fused to SNAP-tag and for direct quantification of SNAP-tagged β-tubulin in cell lysates. In addition, the authors have characterized a fast-labeling variant of SNAP-tag, termed SNAPf, which displays up to a tenfold increase in its reactivity towards benzylguanine substrates. The presented data demonstrate that the combination of SNAPf and the fluorogenic substrates greatly reduces the background fluorescence for labeling and imaging applications. This approach enables highly sensitive spatiotemporal investigation of protein dynamics in living cells.

ChemBioChem published new progress about Cell membrane. 352351-55-4 belongs to class bromides-buliding-blocks, name is (9H-Fluoren-9-yl)methyl (2-aminoethyl)carbamate hydrobromide, and the molecular formula is C17H19BrN2O2, Computed Properties of 352351-55-4.

Referemce:
Bromide – Wikipedia,
bromide – Wiktionary

Hollas, Michael A. published the artcileA Bifunctional Spin Label for Ligand Recognition on Surfaces, Related Products of bromides-buliding-blocks, the main research area is bifunctional spin ligand recognition surfaces; EPR spectroscopy; carbohydrates; nanoparticles; sensors; spin labels.

In situ monitoring of biomol. recognition, especially at surfaces, still presents a significant tech. challenge. ESR of biomols. spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin-labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose-binding lectin, Con A, and the biotin-binding protein avidin-peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

Angewandte Chemie, International Edition published new progress about ESR (electron spin resonance). 352351-55-4 belongs to class bromides-buliding-blocks, name is (9H-Fluoren-9-yl)methyl (2-aminoethyl)carbamate hydrobromide, and the molecular formula is C17H19BrN2O2, Related Products of bromides-buliding-blocks.

Referemce:
Bromide – Wikipedia,
bromide – Wiktionary

Takashima, Hiroshi published the artcileSynthesis and spectroscopic properties of reconstituted zinc-myoglobin appending a DNA-binding platinum(II) complex, COA of Formula: C17H19BrN2O2, the main research area is preparation myoglobin zinc protoporphyrin derivative platinum bipyridine complex; DNA binding myoglobin zinc protoporphyrin derivative platinum bipyridine complex; fluorescence myoglobin zinc protoporphyrin derivative platinum bipyridine complex.

Apo-myoglobin (apo-Mb) was reconstituted with a zinc-porphyrin derivative linked via an ethylenediamine derivative to a DNA-binding Pt complex, [Pt(bpy)(μ-enPP)Zn]Cl2 (μ-enPP = protoporphyrin derivative linked to ethylenediamine, bpy = 2,2′-bipyridine). The steady-state fluorescence of the cofactor, [Pt(bpy)(μ-enPP)Zn]Cl2, in MeOH indicates that the excited singlet state of zinc-porphyrin was almost quenched, probably because of the strong hydrophobic and π-π stacking interactions between the [Pt(bpy)(μ-enPP)Zn]2+ ions. In the reconstituted Mb-[Pt(bpy)(μ-enPP)Zn]2+, the quenching reaction of 1(ZnMb)* with the [Pt(bpy)(en)]2+ moiety does not occur, indicating apo-Mb matrix is essential. However, when the [Pt(bpy)(en)]2+ moiety was excited, the enhancement of the fluorescence from ZnMb unit was observed It is suggested that the energy transfer from 1([Pt(bpy)(en)]2+)* to ZnMb occurs. The spectroscopic changes of ZnMb-[Pt(bpy)(en)]2+ in the presence of calf-thymus DNA were also studied. Soret band at 428 nm gradually decreased, and isosbestic points at 321, 414, and 432 nm were observed with increasing DNA concentration When the PtII moiety was excited at λex 321 nm, the fluorescence signal around 600 nm similarly decreased. The synthetic modification of ZnMb with a DNA-binding PtII complex demonstrates sensitive fluorescent signal for DNA detection and valuable information to study photoinduced electron transfer within a Mb-DNA complex.

Chemistry & Biodiversity published new progress about Fluorescence. 352351-55-4 belongs to class bromides-buliding-blocks, name is (9H-Fluoren-9-yl)methyl (2-aminoethyl)carbamate hydrobromide, and the molecular formula is C17H19BrN2O2, COA of Formula: C17H19BrN2O2.

Referemce:
Bromide – Wikipedia,
bromide – Wiktionary