《A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus》 was written by Grolla, Ambra A.; Miggiano, Riccardo; Marino, Daniele Di; Bianchi, Michele; Gori, Alessandro; Orsomando, Giuseppe; Gaudino, Federica; Galli, Ubaldina; Del Grosso, Erika; Mazzola, Francesca; Angeletti, Carlo; Guarneri, Martina; Torretta, Simone; Calabro, Marta; Boumya, Sara; Fan, Xiaorui; Colombo, Giorgia; Travelli, Cristina; Rocchio, Francesca; Aronica, Eleonora; Wohlschlegel, James A.; Deaglio, Silvia; Rizzi, Menico; Genazzani, Armando A.; Garavaglia, Silvia. Quality Control of 8-Bromooctanoic acid And the article was included in Journal of Biological Chemistry in 2020. The article conveys some information:
All cells require sustained intracellular energy flux, which is driven by redox chem. at the subcellular level. NAD+, its phosphorylated variant NAD(P)+, and its reduced forms NAD(P)/NAD(P)H are all redox cofactors with key roles in energy metabolism and are substrates for several NAD-consuming enzymes (e.g. poly(ADP-ribose) polymerases, sirtuins, and others). The nicotinamide salvage pathway, constituted by NMN adenylyltransferase (NMNAT) and nicotinamide phosphoribosyltransferase (NAMPT), mainly replenishes NAD+ in eukaryotes. However, unlike NMNAT1, NAMPT is not known to be a nuclear protein, prompting the question of how the nuclear NAD+ pool is maintained and how it is replenished upon NAD+ consumption. In the present work, using human and murine cells; immunoprecipitation, pulldown, and surface plasmon resonance assays; and immunofluorescence, small-angle X-ray scattering, and MS-based analyses, we report that GAPDH and NAMPT form a stable complex that is essential for nuclear translocation of NAMPT. This translocation furnishes NMN to replenish NAD+ to compensate for the activation of NAD-consuming enzymes by stressful stimuli induced by exposure to H2O2 or S-nitrosoglutathione and DNAdamage inducers. These results indicate that by forming a complex with GAPDH, NAMPT can translocate to the nucleus and thereby sustain the stress-induced NMN/NAD salvage pathway. In the part of experimental materials, we found many familiar compounds, such as 8-Bromooctanoic acid(cas: 17696-11-6Quality Control of 8-Bromooctanoic acid)
8-Bromooctanoic acid(cas: 17696-11-6) acid is used in the synthesis of 8-(N-Methyl-4,4′-bipyridinyl)- octanoic acid. 8-Mercaptooctanoic acid was prepared from 8-bromooctanoic acid. And 8-Bromooctanoic Acid is a useful compound for sonodynamic therapy.Quality Control of 8-Bromooctanoic acid
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary