COA of Formula: C14H21Br2ClN2In 2020, Dinh, Chung Duong;Thi, Ngoc Yen Nguyen published 《Optimizing the process of bromhexine determination in tablets by using the response surface methodology》. 《Journal of Chemical and Pharmaceutical Research》published the findings. The article contains the following contents:
The study is to establish a method for the determination of bromhexine hydrochloride in tablets. Bromhexine hydrochloride in the tablets were analyzed by a HIQ Sil C18 column (250 mm x 4.6 mm; 5μm) with a mobile phase including methanol -0.09% acid orthophosphoric (60:40, volume/volume). The flow rate was 0.9 mL/min with the detection wavelength at 247 nm. This method was validated according to the International Conference on Harmonisation (ICH) Q2 (R1) guideline. Calibration graph was linear (r = 0.9999, n = 7) in concentration range of 2-40μg/mL. The average recovery of bromhexine hydrochloride was 99.63%, and RSD was 1.01% (n = 12). The simple, fast, and reproducible RP-HPLC method has been successfully developed and validated. The proposed method is used for the quantitation of bromhexine in some products in the market. And 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride (cas: 611-75-6) was used in the research process.
2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride(cas: 611-75-6) (BHH; 250μM; 24 hours) also significantly attenuates HGF-induced invasion of LNCaP and C4-2B cells that natively express TMPRSS2. No significant toxicity is observed over a 48-hour period exposing LNCaP, DU145, PC3, or HepG2 cells to Bromhexine hydrochloride concentrations ranging from 0μM to 250μM. Bromhexine hydrochloride exposure does not induce cell death or substantially suppress the growth of DU145 cells.Bromhexine hydrochloride (20 μM; 48 h) inhibits dendritic cells infection with HIV-1.COA of Formula: C14H21Br2ClN2
Reference:
Bromide – Wikipedia,
bromide – Wiktionary