Development of an indirect competitive enzyme-linked immunosorbent assay for propiconazole based on monoclonal antibody was written by Li, Jiao;Ding, Yuan;Chen, He;Sun, Wanlin;Huang, Yue;Liu, Fengquan;Wang, Minghua;Hua, Xiude. And the article was included in Food Control in 2022.Category: bromides-buliding-blocks This article mentions the following:
Propiconazole is a widely used fungicide in agricultural production, but it poses risks to human health. Here, an indirect competitive ELISA (ic-ELISA) based on a monoclonal antibody (mAb) was established to detect propiconazole residues. Three haptens of propiconazole were designed and synthesized to prepare antigens. Ten mAbs were obtained using hapten B (5-(4-(2-((1H-1,2,4-triazol-1-yl)methyl)-4-propyl-1,3-dioxolan-2-yl)-3-chlorophenyl) pentanoic acid). The ic-ELISA based on the combination of mAb 2G2E12 and hapten B-ovalbumin (coating antigen) showed the highest sensitivity. After optimization, the 50% inhibition concentration, limit of detection and linear range of the ic-ELISA were 2.33 μg/L, 0.26 μg/L and 0.26-21.28 μg/L, resp. The cross-reactivities for the analogs of propiconazole were ≤1.9%. The average recoveries of the spiked samples were 75.7%-117.0% with relative standard deviations of ≤9.4%. The results of the ic-ELISA were consistent with those of gas chromatog.-mass spectrometry in the anal. of blind samples. Therefore, we provide an alternative tool for the accurate and reliable determination of propiconazole. In the experiment, the researchers used many compounds, for example, 1-Bromopyrrolidine-2,5-dione (cas: 128-08-5Category: bromides-buliding-blocks).
1-Bromopyrrolidine-2,5-dione (cas: 128-08-5) belongs to organobromine compounds. Bromo compounds are employed in a variety of metal-catalyzed coupling reactions. They are also ideal candidates for the synthesis of Grignard reagents that have wide-applicability in organic synthesis. Bromine-containing agents predominate because not only are they more efficient than similar chlorine-containing species, but also the high atomic weight of bromine ensures that it is present in a high mass fraction within most organobromine compounds.Category: bromides-buliding-blocks
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary