Improved analytical methodology for the derivatization and HPLC-fluorometric determination of okadaic acid in phytoplankton and shellfish was written by Dickey, Robert W.;Granade, H. Ray;Bencsath, F. Aladar. And the article was included in Developments in Marine Biology in 1993.COA of Formula: C18H11BrO The following contents are mentioned in the article:
A method for the derivatization and liq chromatog. detn of the marine toxin okadaic acid (OA) was developed. OA was extracted from cultured dinoflagellates and from homogenized shellfish with aqueous 80% MeOH and partitioned into chloroform from water. OA in the extract was derivatized in acetonitrile with 1-bromoacetylpyrene. The product, pyrenacyl okadaate, was then separated from excess reagent and reaction byproducts by solid-phase extraction Analyses were carried out in acetonitrile solution on a C18 reversed-phase column using aqueous 75% acetonitrile as mobile phase with fluorometric detection at 365 nm excitation and 418 nm emission. Deoxycholic acid (DOCA) derivatized and extracted by the same procedure was employed as internal standard Peak height coefficients of variation for 12 randomized injections of 3 OA standard concentrations (5, 10, and 50 ng) with fixed DOCA concentrations (40 ng) ranged from 1.72% to 8.65% for OA and 1.82% to 9.23% for DOCA. The relative retention time OA/DOCA was 0.598 (n =13). Standard OA recoveries from shellfish at spike levels of 1.0 ug/g or larger were >95%. The method was linear between 1.0 and 80 ng OA injected and the lower limit of detection was 0.1 ng. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5COA of Formula: C18H11BrO).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Bromine is more electronegative than carbon (2.9 vs 2.5). Consequently, the carbon in a carbon–bromine bond is electrophilic, i.e. alkyl bromides are alkylating agents. Commercially available organobromine pharmaceuticals include the vasodilator nicergoline, the sedative brotizolam, the anticancer agent pipobroman, and the antiseptic merbromin. COA of Formula: C18H11BrO
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary