An, Peng published the artcileSterically Shielded, Stabilized Nitrile Imine for Rapid Bioorthogonal Protein Labeling in Live Cells, Recommanded Product: Methyl 3,5-dibromo-4-methylbenzoate, the main research area is stabilized nitrile imine bioorthogonal protein labeling mammalian cell.
In pursuit of fast bioorthogonal reactions, reactive moieties have been increasingly employed for selective labeling of biomols. in living systems, posing a challenge in attaining reactivity without sacrificing selectivity. To address this challenge, here the authors report a bioinspired strategy in which mol. shape controls the selectivity of a transient, highly reactive nitrile imine dipole. By tuning the shape of structural pendants attached to the ortho position of the N-aryl ring of diaryltetrazoles, precursors of nitrile imines, the authors discovered a sterically shielded nitrile imine that favors the 1,3-dipolar cycloaddition over the competing nucleophilic addition The photogenerated nitrile imine exhibits an extraordinarily long half-life of 102 s in aqueous medium, owing to its unique mol. shape that hinders the approach of a nucleophile as shown by DFT calculations The utility of this sterically shielded nitrile imine in rapid (∼1 min) bioorthogonal labeling of glucagon receptor in live mammalian cells was demonstrated.
Journal of the American Chemical Society published new progress about 1,3-Dipolar cycloaddition reaction. 74896-66-5 belongs to class bromides-buliding-blocks, name is Methyl 3,5-dibromo-4-methylbenzoate, and the molecular formula is C9H8Br2O2, Recommanded Product: Methyl 3,5-dibromo-4-methylbenzoate.
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary