Guo, Pu published the artcileCompound shape effects in minor groove binding affinity and specificity for mixed sequence DNA, HPLC of Formula: 53484-26-7, the publication is Journal of the American Chemical Society (2018), 140(44), 14761-14769, database is CAplus and MEDLINE.
AT-specific heterocyclic cations that bind in the DNA duplex minor groove have had major successes as cell and nuclear stains and as therapeutic agents which can effectively enter human cells. Expanding the DNA sequence recognition capability of the minor groove compounds could also expand their therapeutic targets and have an impact in many areas, such as modulation of transcription factor biol. activity. 2268681509. Success in the design of mixed sequence binding compounds has been achieved with N-methylbenzimidazole (N-MeBI) thiophenes which are preorganized to fit the shape of the DNA minor groove and H-bond to the -NH of G·C base pairs that projects into the minor groove. Initial compounds bound strongly to a single G·C base pair in an AT context with a specificity ratio of 50 (Kd AT-GC/Kd AT) or less and this was somewhat low for biol. use. We felt that modifications of compound shape could be used to probe local DNA microstructure in target mixed base-pair sequences of DNA and potentially improve the compound binding selectivity. Modifications were made by increasing the size of the benzimidazole N-substituent, e.g., by using N-iso-Bu instead of N-Me, and by changing the mol. twist by introducing substitutions at specific positions on the aromatic core of the compounds In both cases, we were able to achieve a dramatic increase in binding specificity, including no detectable binding to pure AT sequences, without a significant loss in affinity to mixed base-pair target sequences.
Journal of the American Chemical Society published new progress about 53484-26-7. 53484-26-7 belongs to bromides-buliding-blocks, auxiliary class Bromide,Nitro Compound,Amine,Benzene, name is 4-Bromo-N-methyl-2-nitroaniline, and the molecular formula is C7H7BrN2O2, HPLC of Formula: 53484-26-7.
Referemce:
https://en.wikipedia.org/wiki/Bromide,
bromide – Wiktionary