A new HPLC analysis of residual penicillins in edible animal tissues by pre-column fluorescence derivatization was written by Ushimizu, Toru;Sato, Toshio;Saito, Tadao;Itoh, Takatoshi. And the article was included in Animal Science Journal (Tokyo, Japan) in 2001.Reference of 80480-15-5 The following contents are mentioned in the article:
An anal. method was developed for the simultaneous determination of penicillins (benzylpenicillin, oxacillin, cloxacillin, dicloxacillin and nafcillin) remaining in edible tissues (muscle and kidney) of animals (bovine and swine) by pre-column fluorescence derivatization. For labeling a carboxyl group in penicillins, 7 kinds of fluorescence derivatization reagents were tested: 3-bromomethyl-6,7-dimethoxy-1-methyl-1,2-dihydroquinoxaline-2-one, 4-bromomethyl-7-methoxycoumarin, 5,6-dimethoxy-2-(4-hydrazinocarbonylphenyl)benzothiazole, 1-bromoacetylpyrene (BAP), 6,7-dimethoxy-1-methyl-2(H)-quinolinone-3-propinohydrazide, 2-(2,3-naphthalimino)ethyl trifluoromethanesulfonate and 9-anthryldiazomethane. BAP showed the best score on reactivity and was selected and used for the following examinations Optimum conditions for HPLC anal. were determined with reference to the stability of each reagent, reactive temperature and time. Edible animal tissues were extracted with water and deproteinized with sulfuric acid and sodium tungstate, followed by concentration with C1 (solid-phase extraction) cartridge column. The acetonitrile eluate from the column was derivatized with BAP and 18-crown-6 reagents at 40°C for 30 min. The derivatized sample was analyzed by HPLC with reversed-phase mode. The mean recovery range was 73-96% for benzylpenicillin, 73- 90% for oxacillin, 64-83% for cloxacillin, 62-71% for nafcillin and 61-67% for dicloxacillin in tissues. The detection limits for benzylpenicillin and other penicillins were about 2 and 5 ng/g min tissues, resp. In the suspected case of penicillin residues, benzylpenicillin was determined (0.045-29.0 μg/g) in bovine muscle and kidney by this method. The simple and sensitive method was expected for routine anal. of penicillins in edible animal tissues. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Reference of 80480-15-5).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Bromine is more electronegative than carbon (2.9 vs 2.5). Consequently, the carbon in a carbon–bromine bond is electrophilic, i.e. alkyl bromides are alkylating agents. In the pharmaceutical industry organo bromine derivatives are used as sedatives, vasodilators, antiseptic agents, and anticancer agents.Reference of 80480-15-5
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary