Determination of dialkyl phosphates as breakdown products of organophosphorus insecticides in fruit juices by HPTLC with fluorescence detection was written by Schwack, Wolfgang;Zeisler, Tatjana;Stiefel, Constanze. And the article was included in Journal of AOAC International in 2009.Name: 2-Bromo-1-(pyren-1-yl)ethanone The following contents are mentioned in the article:
Dialkyl phosphates (DAP) are common degradation products of organophosphorus pesticides that are used as urinary biomarkers for human exposure. An HPTLC method was developed for the quant. determination of DAP in fruit juices, i.e., di-Me phosphate (DMP), di-Me thiophosphate (DMTP), di-Et phosphate (DEP), and di-Et thiophosphate (DETP). Di-Bu phosphate (DBP) was used as an internal standard The method was based on precipitation of fruit acids in the presence of barium chloride and acetonitrile and liquid-liquid extraction with acetonitrile-diethyl ether. Extracted DAP were derivatized with 1-(bromoacetyl)pyrene (BAP), and the BAP derivatives separated on HPTLC amino plates with dichloromethane as the mobile phase. Densitometry was performed by measurement of fluorescence at 366/>400 nm. The limit of quantification (LOQ) values were between 0.8 and 1.4 ng/zone. Fluorescence enhancement was achieved by dipping the plate into a paraffin oil solution, increasing the sensitivity and resulting in and LOQ of 0.5-0.6 ng/zone. Repeatabilities with relative standard deviations of ≤3.5% (n = 5, at 15-20 ng/zone) and coefficients of correlation of 0.9999 were highly satisfactory for rapid trace anal. of DAP in the fruit juices by HPTLC. The mean recoveries from apple juice spiked at 0.5 mg/L were 74, 83, 70, and 57% for DMP, DEP, DMTP, and DETP, resp. If an application volume of 5 μL of apple juice extract was applied, the LOQ in apple juice was 300 μg/L. However, this can be lowered by application of higher volumes (up to 50 μL) or a more concentrated derivatization batch. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Name: 2-Bromo-1-(pyren-1-yl)ethanone).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Most of the natural organobromine compounds are produced by marine organisms, and several brominated metabolites with antibacterial, antitumor, antiviral, and antifungal activity have been isolated from seaweed, sponges, corals, molluscs, and others. When the molecular ion is detected, the bromine and chlorine isotope patterns are very distinct, but caution is to be exercised for certain mixed chlorinated/brominated compounds, which can look similar to homohalogen patterns.Name: 2-Bromo-1-(pyren-1-yl)ethanone
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary