Determination of simvastatin and its active metabolite in human plasma by column-switching high-performance liquid chromatography with fluorescence detection after derivatization with 1-bromoacetylpyrene was written by Ochiai, Hisao;Uchiyama, Naotaka;Imagaki, Kazuhide;Hata, Shunsuke;Kamei, Toshio. And the article was included in Journal of Chromatography B: Biomedical Sciences and Applications in 1997.Application In Synthesis of 2-Bromo-1-(pyren-1-yl)ethanone The following contents are mentioned in the article:
By using a fluorescent derivatization and column-switching technique, a highly sensitive and selective high-performance liquid chromatog. (HPLC) method has been developed for the determination of simvastatin (I, β-hydroxy-δ-lactone form) and its active hydrolyzed metabolite (II, β,δ-dihydroxy acid form of I) in human plasma. A plasma sample spiked with internal standards was applied to a C8 solid-phase extraction column. I and II were sep. extracted from internal standards was applied to a C8 solid-phase extraction column. I and II were sep. extracted from plasma into two fractions. I in one of the fractions was hydrolyzed to II. A fluorescent derivative was prepared by esterification of II with 1-bromoacetylpyrene in the presence of 18-crown-6 for both fractions. The pyrenacyl ester of II thus obtained was purified on a phenylboronic acid (PBA) solid-phase extraction column, and was measured by column-switching HPLC with fluorescence detection. The calibration curves for both I and II were linear in the concentration range of 0.1-10 ng/mL. The intra-day coefficients of variation were less than 11.0%, and the accuracies were between 91.7% and 117% within the concentration range for both analytes. The limits of quantification (LOQ) for both analytes were set to 0.1 ng/mL. This assay method has adequate sensitivity and selectivity to measure the concentrations of I and II in human plasma from clin. studies. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Application In Synthesis of 2-Bromo-1-(pyren-1-yl)ethanone).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Many of the organo bromine compounds are relatively nonpolar. Bromine is more electronegative than carbon (2.8 vs 2.5) and hence the carbon in a carbonbromine bond is electrophilic in nature. When the molecular ion is detected, the bromine and chlorine isotope patterns are very distinct, but caution is to be exercised for certain mixed chlorinated/brominated compounds, which can look similar to homohalogen patterns.Application In Synthesis of 2-Bromo-1-(pyren-1-yl)ethanone
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary