Fluorescence high-performance liquid chromatographic determination of bile acids and its application to fecal bile acids was written by Miyake, Mariko;Mura, Tetsuo;Yurino, Nobuo;Shinzawa, Takeshi;Ikawa, Shiro. And the article was included in Yonago Igaku Zasshi in 1986.Safety of 2-Bromo-1-(pyren-1-yl)ethanone The following contents are mentioned in the article:
Free bile acids were labeled with 1-bromoacetylpyrene in MeCN by using dicyclohexyl-18-crown-6-ether as catalyst. Fluorescent derivatives of bile acids were analyzed on a reversed-phase column (Shim-pack CLC-ODS), using MeCN-MeOH-H2O as mobile phase. Fluorescence intensity was linear from 25 to 400 pmol for 5 common bile acids. Total fecal bile acid level was 9654 ± 3652 μg/g dry feces in healthy men and 9091 ± 4275 μg/g dry feces in healthy women. Major free bile acids in feces were deoxycholic acid (24.5%), β-lithocholic acid (10.3%), lithocholic acid (10.2%), 3β,12α-dihydroxycholanoic acid (7.4%), chenodeoxycholic acid (6.9%), 3α,12β-dihydroxycholanoic acid (6.7%), 12α-hydroxy-3-ketocholanoic acid (5.5%), 3-ketocholanoic acid (4.5%), 3α,7α-dihydroxy-12-ketocholanoic acid (4.4%), and cholic acid (3.7%). This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Safety of 2-Bromo-1-(pyren-1-yl)ethanone).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Bromo compounds are employed in a variety of metal-catalyzed coupling reactions. They are also ideal candidates for the synthesis of Grignard reagents that have wide-applicability in organic synthesis. alpha-Bromoesters are employed in the Reformatsky reaction for the synthesis of beta-hydroxyesters. One prominent application of synthetic organobromine compounds is the use of polybrominated diphenyl ethers as fire-retardants, and in fact fire-retardant manufacture is currently the major industrial use of the element bromine.Safety of 2-Bromo-1-(pyren-1-yl)ethanone
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary