Luminescent Palladacycles Containing a Pyrene Chromophor; Synthesis, Biological and Computational Studies of the Interaction with DNA and BSA was written by Karami, Kazem;Ramezanpour, Azar;Zakariazadeh, Mostafa;Shahpiri, Azar;Kharaziha, Mahshid;Kazeminasab, Akram. And the article was included in ChemistrySelect in 2019.Quality Control of 2-Bromo-1-(pyren-1-yl)ethanone The following contents are mentioned in the article:
One of the most active areas within the field of bioorganometallic chem., complexes of N-heterocyclic carbenes (NHCs), have recently gained interest. Herein, we report two luminescent palladium N-heterocyclic carbene complexes; namely [Pd{(C,N)-C6H4CH2NH(CH2CH3)}(1)] (2) and [Pd{(C,N)-C6H4CH2NH2}(1)] (3) (1=1-methyl-3-(2-oxo-2-(pyren-1-yl)ethyl)-2,3-dihydroimidazol-2-ylidene) which were synthesized from the reaction of luminescent imidazolium salt (1(H)Br) and binuclear Palladacycles. The interactions of them with CT-DNA evaluated via absorption, emission and CD spectral techniques as well as measurements of viscosity and thermal denaturation and the results have been shown that they bounded to CT-DNA by intercalation and groove binding modes. The in vitro cytotoxicity of compounds 2–3 and 1(H)Br on human breast (MCF-7) and cervical epithelial carcinoma (HeLa) cancer cells lines, indicated the wide range of anticancer activities of them with low IC50 values. Moreover, based on the protein binding ability studies, the intrinsic fluorescence of BSA could be strongly quenched by compounds via a static quenching mechanism. Competitive binding study using Eosin, Digoxin and Ibuprofen as site markers, indicated that the compounds could bind to sites I and II on BSA structure. Finally, all data obtained from biophys. studies were validated by mol. modeling study. Computational results showed that palladium complexes have the potential for detection of mismatch DNA. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Quality Control of 2-Bromo-1-(pyren-1-yl)ethanone).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Bromo compounds are employed in a variety of metal-catalyzed coupling reactions. They are also ideal candidates for the synthesis of Grignard reagents that have wide-applicability in organic synthesis. alpha-Bromoesters are employed in the Reformatsky reaction for the synthesis of beta-hydroxyesters. Commercially available organobromine pharmaceuticals include the vasodilator nicergoline, the sedative brotizolam, the anticancer agent pipobroman, and the antiseptic merbromin. Quality Control of 2-Bromo-1-(pyren-1-yl)ethanone
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary