Briand, Loic published the artcileCharacterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier, HPLC of Formula: 56523-59-2, the main research area is chemosensory protein sequence characterization honey bee.
We report the cloning of a honeybee chemosensory proteins (CSP) gene called ASP3c, as well as the structural and functional characterization of the encoded protein. The protein was heterologously secreted by the yeast Pichia pastoris using the native signal peptide. ASP3c disulfide bonds were assigned after trypsinolysis followed by chromatog. and mass spectrometry combined with microsequencing. The pairing (Cys(I)-Cys(II), Cys(III)-Cys(IV)) was found to be identical to that of Schistocerca gregaria CSPs, suggesting that this pattern occurs commonly throughout the insect CSPs. CD measurements revealed that ASP3c mainly consists of α-helixes, like other insect CSPs. Gel filtration anal. showed that ASP3c is monomeric at neutral pH. Using ASA, a fluorescent fatty acid anthroyloxy analog as a probe, ASP3c was shown to bind specifically to large fatty acids and ester derivatives, which are brood pheromone components, in the micromolar range. It was unable to bind tested general odorants and other tested pheromones (sexual and nonsexual). This is the 1st report on a natural pheromonal ligand bound by a recombinant CSP with a measured affinity constant
European Journal of Biochemistry published new progress about Antenna (anatomical). 56523-59-2 belongs to class bromides-buliding-blocks, name is 15-Bromopentadecanoic acid, and the molecular formula is C15H29BrO2, HPLC of Formula: 56523-59-2.
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary