《SNP discrimination by tolane-modified peptide nucleic acids: application for the detection of drug resistance in pathogens》 was written by Takagi, Kenji; Hayashi, Tenko; Sawada, Shinjiro; Okazaki, Miku; Hori, Sakiko; Ogata, Katsuya; Kato, Nobuo; Ebara, Yasuhito; Kaihatsu, Kunihiro. Name: Methyl 3-bromopropanoateThis research focused onzanamivir oseltamivir antiviral agent PNA drug resistance SNP influenza; drug resistance; influenza virus; peptide nucleic acid; single nucleotide polymorphism; tolane. The article conveys some information:
During the treatment of viral or bacterial infections, it is important to evaluate any resistance to the therapeutic agents used. An amino acid substitution arising from a single base mutation in a particular gene often causes drug resistance in pathogens. Therefore, mol. tools that discriminate a single base mismatch in the target sequence are required for achieving therapeutic success. Here, we synthesized peptide nucleic acids (PNAs) derivatized with tolane via an amide linkage at the N-terminus and succeeded in improving the sequence specificity, even with a mismatched base pair located near the terminal region of the duplex. We assessed the sequence specificities of the tolane-PNAs for single-strand DNA and RNA by UV-melting temperature anal., thermodn. anal., an in silico conformational search, and a gel mobility shift assay. As a result, all of the PNA-tolane derivatives stabilized duplex formation to the matched target sequence without inducing mismatch target binding. Among the different PNA-tolane derivatives, PNA that was modified with a naphthyl-type tolane could efficiently discriminate a mismatched base pair and be utilized for the detection of resistance to neuraminidase inhibitors of the influenza A/H1N1 virus. Therefore, our mol. tool can be used to discriminate single nucleotide polymorphisms that are related to drug resistance in pathogens. After reading the article, we found that the author used Methyl 3-bromopropanoate(cas: 3395-91-3Name: Methyl 3-bromopropanoate)
Methyl 3-bromopropanoate(cas: 3395-91-3) belongs to bromides. One prominent application of synthetic organobromine compounds is the use of polybrominated diphenyl ethers as fire-retardants, and in fact fire-retardant manufacture is currently the major industrial use of the element bromine.Name: Methyl 3-bromopropanoate
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary