The author of 《Broad-spectrum monoclonal antibody and a sensitive multi-residue indirect competitive enzyme-linked immunosorbent assay for the antibacterial synergists in samples of animal origin》 were Han, Xiaoya; Sheng, Feng; Kong, Dexin; Wang, Yulian; Pan, Yuanhu; Chen, Mo; Tao, Yanfei; Liu, Zhenli; Ahmed, Saeed; Yuan, Zonghui; Peng, Dapeng. And the article was published in Food Chemistry in 2019. Application of 2969-81-5 The author mentioned the following in the article:
To monitor the abuse of antibacterial synergists, a hapten, trimethoprim carboxylic derivative (TMPCOOH), was designed by using mol. modeling technol. A broad-spectrum monoclonal antibody (mAb) TMP/2G1 was prepared, for which the IC50 values of trimethoprim, diaveridine, aditoprim, baquiloprim, ormetoprim, and brodimoprim were 0.232, 0.527, 1.479, 4.354, 0.965, and 0.119 μg L-1, resp. Based on the broad spectrum mAb, an indirect competitive ELISA (ic-ELISA) was developed to determine the residues of antibacterial synergists. The limit of detection regarding the developed ic-ELISA for antibacterial synergists ranged from 0.025 to 1.126 μg L-1 in milk, honey and edible animal tissues. The recoveries ranged from 81.4% to 107.7%, with a coefficient of variation less than 20%. A good correlation (R2 = 0.994) between the ic-ELISA and HPLC-MS/MS showed the reliability of the developed ic-ELISA. In the experiment, the researchers used many compounds, for example, Ethyl 4-bromobutyrate(cas: 2969-81-5Application of 2969-81-5)
Ethyl 4-bromobutyrate(cas: 2969-81-5) belongs to bromides. A variety of minor organobromine compounds are found in nature, but none are biosynthesized or required by mammals. Organobromine compounds have fallen under increased scrutiny for their environmental impact.Application of 2969-81-5
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary