《A Click-Chemistry-Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry》 was published in ChemBioChem in 2020. These research results belong to Stadlmeier, Michael; Runtsch, Leander Simon; Streshnev, Filipp; Wuehr, Martin; Carell, Thomas. Safety of Methyl 3-bromopropanoate The article mentions the following:
Mass spectrometry is the method of choice for the characterization of proteomes. Most proteins operate in protein complexes, in which their close association modulates their function. However, with standard MS anal., information on protein-protein interactions is lost and no structural information is retained. To gain structural and interactome data, new crosslinking reagents are needed that freeze inter- and intramol. interactions. Herein, the development of a new reagent, which has several features that enable highly sensitive crosslinking MS, is reported. The reagent enables enrichment of crosslinked peptides from the majority of background peptides to facilitate efficient detection of low-abundant crosslinked peptides. Due to the special cleavable properties, the reagent can be used for MS2 and potentially for MS3 experiments Thus, the new crosslinking reagent, in combination with high-end MS, should enable sensitive anal. of interactomes, which will help researchers to obtain important insights into cellular states in health and diseases. In addition to this study using Methyl 3-bromopropanoate, there are many other studies that have used Methyl 3-bromopropanoate(cas: 3395-91-3Safety of Methyl 3-bromopropanoate) was used in this study.
Methyl 3-bromopropanoate(cas: 3395-91-3) belongs to bromides. Most organobromine compounds, like most organohalide compounds, are relatively nonpolar. Bromine is more electronegative than carbon (2.9 vs 2.5). Consequently, the carbon in a carbon–bromine bond is electrophilic, i.e. alkyl bromides are alkylating agents.Safety of Methyl 3-bromopropanoate
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary