In 2018,Bioconjugate Chemistry included an article by Veerapen, Natacha; Kharkwal, Shalu Sharma; Jervis, Peter; Bhowruth, Veemal; Besra, Amareeta K.; North, Simon J.; Haslam, Stuart M.; Dell, Anne; Hobrath, Judith; Quaid, Padraic J.; Moynihan, Patrick J.; Cox, Liam R.; Kharkwal, Himanshu; Zauderer, Maurice; Besra, Gurdyal S.; Porcelli, Steven A.. HPLC of Formula: 17696-11-6. The article was titled 《Photoactivable Glycolipid Antigens Generate Stable Conjugates with CD1d for Invariant Natural Killer T Cell Activation》. The information in the text is summarized as follows:
Activation of invariant natural killer T lymphocytes (iNKT cells) by α-galactosylceramide (α-GC) elicits a range of pro-inflammatory or anti-inflammatory immune responses. We report the synthesis and characterization of a series of α-GC analogs with acyl chains of varying length and a terminal benzophenone. These bound efficiently to the glycolipid antigen presenting protein CD1d, and upon photoactivation formed stable CD1d-glycolipid covalent conjugates. Conjugates of benzophenone α-GCs with soluble or cell-bound CD1d proteins retained potent iNKT cell activating properties, with biol. effects that were modulated by acyl chain length and the resulting affinities of conjugates for iNKT cell antigen receptors. Anal. by mass spectrometry identified a unique covalent attachment site for the glycolipid ligands in the hydrophobic ligand binding pocket of CD1d. The creation of covalent conjugates of CD1d with α-GC provides a new tool for probing the biol. of glycolipid antigen presentation, as well as opportunities for developing effective immunotherapeutics. In the experiment, the researchers used many compounds, for example, 8-Bromooctanoic acid(cas: 17696-11-6HPLC of Formula: 17696-11-6)
8-Bromooctanoic acid(cas: 17696-11-6) acid is used in the synthesis of 8-(N-Methyl-4,4′-bipyridinyl)- octanoic acid. 8-Mercaptooctanoic acid was prepared from 8-bromooctanoic acid.HPLC of Formula: 17696-11-6
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary