On October 15, 2021, there was a patent about enzymes.Reference of 3-Bromo-5-fluoro-4-hydroxybenzaldehyde The title of the patent was Fluorescent dye, preparation method therefor and use thereof. And the patent contained the following:
A fluorescent dye I [wherein Ar = (un)substituted arylene or heteroarylene; Y = O or S; R1 = H or alkyl; R2 = halo, OH, or CN], a preparation method therefor and use thereof. For example, II was prepared in a multi-step synthesis. The fluorescent dye has the advantages of sensitivity and specificity to viscosity response, low background fluorescence and the like, and can also be used as a fluorescence activation lighting-type probe for fluorescence labeling, quantification or detection of proteins, enzymes or nucleic acids. The experimental process involved the reaction of 3-Bromo-5-fluoro-4-hydroxybenzaldehyde(cas: 185345-46-4).Reference of 3-Bromo-5-fluoro-4-hydroxybenzaldehyde
The Article related to fluorescent dye preparation fluorescence probe proteins enzyme nucleic acid, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Reference of 3-Bromo-5-fluoro-4-hydroxybenzaldehyde
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary