HPLC of Formula: 586-76-5In 2022 ,《SCP-7, a germacrane-type sesquiterpene lactone derivative, induces ROS-mediated apoptosis in NSCLC cells in vitro and in vivo》 was published in European Journal of Pharmacology. The article was written by Zhang, Yang-Yang; Ren, Hui; Yan, Qiu-Lin; Li, Ya-Ling; Liu, Qingbo; Yao, Guo-Dong; Song, Shao-Jiang. The article contains the following contents:
Scabertopin (SCP), an abundant germacrane-type sesquiterpene lactone (SLC) isolated from Elephantopus scaber, was selected as a reference compound for modification and evaluation as anticancer agents for non-small cell lung cancer (NSCLC) treatment. All derivatives (SCP-1-SCP-13) except for SCP-3 showed potential inhibitory effect (IC50 5.2-9.7 μM) against A549 cells. The most promising compound SCP-7 also showed good cytotoxic activity against another two NSCLC cell lines (H1299 and H460), with IC50 value of 4.4 and 8.9 μM, resp. Furthermore, SCP-7 could induce apoptotic cell death that was associated with the increased reactive oxygen species (ROS) generation, the loss of mitochondrial membrane potential, Bcl-2 family proteins modulation, caspases-3 and PARP cleavage. In addition, SCP-7 also inhibited cell growth by increasing Bax expression and reducing the Ki-67 pos. cells in vivo, but there were no obvious toxic and side effects on internal organs. Mechanistically, PharmMapper, mol. docking and Western blot anal. revealed that SCP-7 might interact with the epidermal growth factor receptor (EGFR) and inhibit its expression in lung cancer cells. Together, above results suggest further effective application of SCP-7 as a potential anti-tumor agent in the treatment of NSCLC. After reading the article, we found that the author used 4-Bromobenzoic acid(cas: 586-76-5HPLC of Formula: 586-76-5)
4-Bromobenzoic acid(cas: 586-76-5) has been used to study the metabolic fate of 2-,3-and 4-bromo benzoic acids in rat hepatocytes incubation using high temperature liquid chromatography. It was used in bromine-specific detection of the metabolites of 2-,3-and 4-bromobenzoic acid in the urine and bile of rats by inductively coupled plasma mass spectrometry.HPLC of Formula: 586-76-5
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary