In 2022,Yamatsugu, Kenzo; Katoh, Hiroto; Yamashita, Takefumi; Takahashi, Kazuki; Aki, Sho; Tatsumi, Toshifumi; Kaneko, Yudai; Kawamura, Takeshi; Miura, Mai; Ishii, Masazumi; Ohkubo, Kei; Osawa, Tsuyoshi; Kodama, Tatsuhiko; Ishikawa, Shumpei; Kanai, Motomu; Sugiyama, Akira published an article in Protein Expression and Purification. The title of the article was 《Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system》.Safety of Benzyl 2-bromoacetate The author mentioned the following in the article:
Antibody-drug conjugates (ADCs) are a major therapeutic tool for the treatment of advanced cancer. Malignant cells in advanced cancer often display multiple genetic mutations and become resistant to monotherapy. Therefore, a therapeutic regimen that simultaneously targets multiple mols. with multiple payloads is desirable. However, the development of ADCs is hampered by issues in biopharmaceutical manufacturing and the complexity of the conjugation process of low-mol.-weight payloads to biologicals. Here, we report antibody mimetic-drug conjugates (AMDCs) developed by exploiting the non-covalent binding property of payloads based on high-affinity binding of mutated streptavidin and modified iminobiotin. Miniprotein antibodies were fused to a low immunogenic streptavidin variant, which was then expressed in Escherichia coli inclusion bodies, solubilized, and refolded into functional tetramers. The AMDC developed against human epidermal growth factor receptor 2 (HER2) effectively killed cultured cancer cells using bis-iminobiotin conjugated to photo-activating silicon phthalocyanine. The HER2-targeting AMDC was also effective in vivo against a mouse KPL-4 xenograft model. This AMDC platform provides rapid, stable, and high-yield therapeutics against multiple targets. In the part of experimental materials, we found many familiar compounds, such as Benzyl 2-bromoacetate(cas: 5437-45-6Safety of Benzyl 2-bromoacetate)
Benzyl 2-bromoacetate(cas: 5437-45-6) belongs to benzyl acetate. Benzyl acetate is an aromatic chemical, usually appearing as a clear liquid with a moderate sweet-jasmine fragrance. This compound appears as a component of some of our fragrance blends.Safety of Benzyl 2-bromoacetate
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary