Tu, Yujie; Yu, Yeqing; Zhou, Zhibiao; Xie, Sheng; Yao, Bicheng; Guan, Shujuan; Situ, Bo; Liu, Yong; Kwok, Ryan T. K.; Lam, Jacky W. Y.; Chen, Sijie; Huang, Xuhui; Zeng, Zebing; Tang, Ben Zhong published the artcile< Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens>, Reference of 184239-35-8, the main research area is tetrazolate functionalized fluorescent probe aggregation induced emission albumin; aggregation-induced emission; albumin; diagnostic detection; tetrazolate−lysine interaction; water-soluble fluorescent probe.
The anal. of albumin has clin. significance in diagnostic tests and obvious value to research studies on the albumin-mediated drug delivery and therapeutics. The present immunoassay, instrumental techniques, and colorimetric methods for albumin detection are either expensive, troublesome, or insensitive. Herein, a class of water-soluble tetrazolate-functionalized derivatives with aggregation-induced emission (AIE) characteristics is introduced as novel fluorescent probes for albumin detection. They can be selectively lighted up by site-specific binding with albumin. The resulting albumin fluorescent assay exhibits a low detection limit (0.21 nM), high robustness in aqueous buffer (pH = 6-9), and a broad tunable linear dynamic range (0.02-3000 mg/L) for quantification. The tetrazolate functionality endows the probes with a superior water solubility (>0.01 M) and a high binding affinity to albumin (KD = 0.25μM). To explore the detection mechanism, three unique polar binding sites on albumin are computationally identified, where the multivalent tetrazolate-lysine interactions contribute to the tight binding and restriction of the mol. motion of the AIE probes. The key role of lysine residues is verified by the detection of poly-L-lysine. Moreover, we applied the fluorogenic method to quantify urinary albumin in clin. samples and found it a feasible and practical strategy for albumin anal. in complex biol. fluids.
ACS Applied Materials & Interfaces published new progress about Aggregation-induced emission. 184239-35-8 belongs to class bromides-buliding-blocks, and the molecular formula is C26H18Br2, Reference of 184239-35-8.
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary