Wan, Nan-Wei; Cui, Hai-Bo; Zhao, Ling; Shan, Jing; Chen, Ke; Wang, Zhong-Qiang; Zhou, Xiao-Jian; Cui, Bao-Dong; Han, Wen-Yong; Chen, Yong-Zheng published the artcile< Directed evolution of cytochrome P450DA hydroxylase activity for stereoselective biohydroxylation>, Formula: C8H9Br, the main research area is chiral alc preparation stereoselective biohydroxylation cytochrome P450DA hydroxylase.
Engineering of a hydroxylase for highly active and stereoselective biohydroxylation of C(sp3)-H bonds attracts keen interest in synthetic chem. Herein, we report the development of a colorimetric high throughput screening assay for the directed evolution of cytochrome P450DA hydroxylase. The best triple-mutant P450DA-M3 (N190F/V356L/A486E) was identified with improvements in the turnover frequency (TOF) and total turnover number (TTN). P450DA-M3 exhibited good catalytic efficiency (up to 6750 TTNs) and stereoselectivity (up to 98% ee) in the biohydroxylation of diverse substrates. The heme domain structure of the P450DA was also solved for understanding the possible influence of these pos. mutations.
Catalysis Science & Technology published new progress about Aralkyl alcohols Role: BPN (Biosynthetic Preparation), BIOL (Biological Study), PREP (Preparation). 2725-82-8 belongs to class bromides-buliding-blocks, and the molecular formula is C8H9Br, Formula: C8H9Br.
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary