Rele, Rajan V. published 《Simultaneous UV-spectrophotometric estimation of bromhexine hydrochloride and salbutamol sulphate by first order derivative method in combined dosage form》 in 2015. The article was appeared in 《Journal of Chemical and Pharmaceutical Research》. They have made some progress in their research.HPLC of Formula: 611-75-6 The article mentions the following:
The objective of the study was to develop a simple, accurate, precise and rapid UV spectrophotometric, first order derivative, method for simultaneous estimation of bromhexine hydrochloride and salbutamol sulfate from combined dosage form. The validation was carried out by using ICH guidelines for the determination of bromhexine hydrochloride and salbutamol sulfate by using 0.1N hydrochloric acid as the solvent in pharmaceutical dosage form. The proposed first order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 240 nm and 233 nm were selected for the estimation of bromhexine hydrochloride and salbutamol sulfate resp. The linearity of the proposed method was found in the concentration range of 2 to 14 μg /mL (r2= 0.9995) for bromhexine hydrochloride and 2 to 16 μg /mL (r2 = 0.9996) for salbutamol sulfate resp. The percentage mean recovery was found to be 100.166 % for bromhexine hydrochloride and 100.147 % for salbutamol sulfate resp. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method. And 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride (cas: 611-75-6) was used in the research process.
2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride(cas: 611-75-6) is a mucolytic agent that exerts both a secretagogic action on submucosal glands and a mucolytic action toward acid glycoproteins inside cells. Bromhexine Hydrochloride is a medication prescribed for coughs which works by dissolving hard phlegm. OthersBromhexine is a mucolytic agent used in the treatment of respiratory disorders associated with viscid or excessive mucus. In addition, bromhexine has antioxidant properties. HPLC of Formula: 611-75-6
Reference:
Bromide – Wikipedia,
bromide – Wiktionary