Mehta, P. S.;Patel, V. B. published 《RP-HPLC method for simultaneous estimation of bromohexine hydrochloride, dextromethorphan hydrobromide and guaiphenesin in soft gel capsules》 in 2013. The article was appeared in 《Asian Journal of Chemistry》. They have made some progress in their research.HPLC of Formula: 611-75-6 The article mentions the following:
A simple, precise, and accurate reverse phase high performance liquid chromatog. method was developed for simultaneous determination of guaiphenesin, dextromethorphan hydrobromide and bromhexine hydrochloride in their soft gel formulations. The chromatog. conditions were standardized using Phenomenex Luna C18 (250 mm × 4.6 mm i.d., 5 μm particle size) with UV detection at 258 nm and mobile phase consisting of methanol: 0.05 M phosphate buffer pH 3.0 (60 : 40 volume/volume). Mobile phase was delivered at the flow rate of 1.3 mL/min and separation was completed within 8 min. The retention times of guaiphenesin, dextromethorphan hydrobromide and bromhexine hydrochloride were 3.1 min, 4.1 min and 6.8 min resp. Calibration curves were linear with correlation coefficient 0.998. 0.999 And 0.999 over a concentration range of 50-250, 5-25 and 1-6 μg/mL for guaiphenesin, dextromethorphan hydrobromide and bromhexine hydrochloride resp. Recoveries were between 97.04-100.15, 101.92-104.67 and 102.01-102.55 resp. The proposed method was validated and successfully applied to the determination of 3 drugs in combined soft gel formulation. The experimental procedure involved many compounds, such as 2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride (cas: 611-75-6) .
2,4-Dibromo-6-((cyclohexyl(methyl)amino)methyl)aniline hydrochloride(cas: 611-75-6) (BHH; 250μM; 24 hours) also significantly attenuates HGF-induced invasion of LNCaP and C4-2B cells that natively express TMPRSS2. No significant toxicity is observed over a 48-hour period exposing LNCaP, DU145, PC3, or HepG2 cells to Bromhexine hydrochloride concentrations ranging from 0μM to 250μM. Bromhexine hydrochloride exposure does not induce cell death or substantially suppress the growth of DU145 cells.Bromhexine hydrochloride (20 μM; 48 h) inhibits dendritic cells infection with HIV-1.HPLC of Formula: 611-75-6
Reference:
Bromide – Wikipedia,
bromide – Wiktionary