Enzymes are biological catalysts that produce large increases in reaction rates and tend to be specific for certain reactants and products, Computed Properties of C18H30O2, 506-26-3, Name is (6Z,9Z,12Z)-Octadeca-6,9,12-trienoic acid, molecular formula is C18H30O2, belongs to bromides-buliding-blocks compound. In a document, author is Ma, Donghui, introduce the new discover.
PLCG2 promotes hepatocyte proliferation in vitro via NF-kappa B and ERK pathway by targeting bcl2, myc and ccnd1
Phospholipase C gamma 2 (PLCG2) has been implicated in the regulation of cell proliferation, transformation, and tumor growth. In this study, we investigate the mechanism of PLCG2 action using a short interference RNA (siRNA) method. The effects of PLCG2 on rat liver BRL-3A cells treated siRNA were studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT assay), bromodeoxyuridine (BrdU) labelling assay, flow cytometry method (FCM), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The results showed when PLCG2 was reduced, cell vitality and proliferation rate were significantly decreased (p < .05 vs. control). FCM analysis showed that the number of cell division phase (G2 + M) was declined (p < .05 vs. control). RT-PCR and western blot revealed that the expression of signalling related genes NF-kappa B, FOS, JUN and ELK, target genes BCL2, CCNB1 and CCND1 were remarkably down-regulated in cells treated with PLCG2 siRNAs. Based on these results, we conclude PLCG2 plays an important role in rat liver cell proliferation via ERK and NF-kappa B pathway by regulating the expression of BCl2, MYC and CCND1. Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law. In my other articles, you can also check out more blogs about 506-26-3. Computed Properties of C18H30O2.