Catalysts are substances that increase the reaction rate of a chemical reaction without being consumed in the process. 108-85-0, Name is Bromocyclohexane, SMILES is BrC1CCCCC1, belongs to bromides-buliding-blocks compound. In a document, author is Yuan, Keyu, introduce the new discover, Product Details of 108-85-0.
MicroRNA-485-5p reduces keratinocyte proliferation and migration by regulating ITGA5 expression in skin wound healing
Purpose: To determine the effect of miR-485-5p on keratinocyte proliferation and migration. Methods: Human primary keratinocytes (HaCaT cells) were treated with different concentrations of transforming growth factor-beta 1 (TGF)-beta 1. miR-485-5p expression levels were determined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) and wound healing assays were performed to investigate the regulatory effects of miR-485-5p on cell viability and migration of HaCaT cells. Downstream target gene expression of miR-485-5p was determined using a luciferase activity assay. Results: In HaCaT cells, miR-485-5p was time- and dose-dependently downregulated by TGF-beta 1 treatment (p < 0.05). Forced expression of miR-485-5p decreased cell viability and migration of HaCaT cells (p < 0.05). Knockdown of miR-485-5p enhanced HaCaT cell viability and migration. Integrin subunit alpha-5 (ITGA5) was predicted and verified to be a downstream target of miR-485-5p in HaCaT cells. Overexpression of ITGA5 attenuated the miR-485-5p-induced decrease of HaCaT cell viability and migration (p < 0.05). Conclusion: MiR-485-5p reduces cell proliferation and migration of keratinocytes through the regulation of ITGA5. This mechanism provides a potential therapeutic strategy for skin wound healing. The proportionality constant is the rate constant for the particular unimolecular reaction. the reaction rate is directly proportional to the concentration of the reactant. I hope my blog about 108-85-0 is helpful to your research. Product Details of 108-85-0.