Chemistry is traditionally divided into organic and inorganic chemistry. The former is the study of compounds containing at least one carbon-hydrogen bonds. 98475-07-1, Name is Methyl 2-(bromomethyl)-3-nitrobenzoate, molecular formula is C9H8BrNO4, belongs to bromides-buliding-blocks compound, is a common compound. In a patnet, author is Goyal, Mehendi, once mentioned the new application about 98475-07-1, HPLC of Formula: C9H8BrNO4.
Hetero-Polysaccharides Secreted from Dunaliella salina Exhibit Immunomodulatory Activity Against Peripheral Blood Mononuclear Cells and RAW 264.7 Macrophages
Several species of microalgae have been known to produce exopolysaccharides (EPS) with potential immune activity. In the present investigation, ethyl acetate fraction of crude EPS secreted by Dunaliella salina was explored for immunomodulatory activity against peripheral blood mononuclear cells (PBMC) and RAW 264.7 macrophages. Effect of EPS on cell growth and cytokines production were measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and ELISA respectively. Griess reagent was used for measuring the nitric oxide production in RAW 264.7 macrophages. FTIR analysis and mass spectroscopy were carried out for the characterization. Ethyl acetate fraction exhibited dose dependent increase in proliferative index and cytokines production (IFN-gamma, TNF-alpha, TGF-beta). At low concentration (250 and 500 mu g/mL), it showed growth inhibition and at higher concentration (1000 and 1500 mu g/mL), it enhanced the cell growth. Interestingly, the pronounced increased TNF-alpha production was observed in ethyl acetate fraction treated PBMC cells at higher concentration (750 and 1000 mu g/mL) indicating the immunostimulatory effect. In RAW cells, concentration dependent diminished cell growth (IC50 = 691 mu g/mL) and nitric oxide production (IC50 = 630 mu g/mL) was observed. FTIR analysis showed the presence of polysaccharides due to the detection of hydroxyl (-OH), Carbonyl (C-O) and alkyl (C-H) groups. Mass spectroscopy results revealed ethyl acetate fraction as penta-saccharide (m/z = 887.56 and 886.54) which are confirmed to be hetero-polysaccharides consisting of hexoses and pentoses along with association of ions. These results suggest that penta-saccharide (ethyl acetate fraction) isolated from D. salina may have the potential to be used for therapeutic purpose as immunomodulatory agent.
We¡¯ll also look at important developments in the pharmaceutical industry because understanding organic chemistry is important in understanding health, medicine, 98475-07-1. The above is the message from the blog manager. HPLC of Formula: C9H8BrNO4.